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Friday, 9 October

NEET UG 2024 · Zoology · Question 169

NEET 2024 Zoology, question 169

Question 169 of the NEET 2024 paper (code T2) is a Zoology question from Biotechnology: Principles and Processes. Its idea, Origin of Replication (ori), was asked 4 times, in 2020, 2024 and 2026, across NEET 2017–2026. The NTA final answer key gives option 4.

Updated 2026-10-08

Q169 · Zoology

The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:

The figure, described in words

Circular cloning vector pBR322 with sites EcoR I, Cla I, Hind III, Pvu I, Pst I, BamH I, Sal I, Pvu II; genes/regions X and Y marked on the circle

  1. 1The gene 'X' is for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
  2. 2Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.
  3. 3The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.
  4. 4The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.Official answer

Official answer: option 4 · NTA final answer key

Same idea

Origin of Replication (ori), in other papers

NEET asked this idea 4 times, in 2020, 2024 and 2026. The other 3 questions below.

  • 2026 Q180Match List-I with List-II. List-I | List-II A. Transformation | I. Restriction enzyme B. Cloning site | II. Transfer DNA to host bacteria…Match the lists · Easy
  • 2024 Q133What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would…Other · Medium
  • 2020 Q78The sequence that controls the copy number of the linked DNA in the vector, is termed :One-line MCQ · Easy

Origin of Replication (ori): every question and how it was framed

The chapter

Biotechnology: Principles and Processes in ten years of NEET

46 questions from 2017–2026, asked in 10 of 10 papers, about 4.4 a paper. Cloning Vectors alone had 18.

Biotechnology: Principles and Processes: questions per paper

0365’171’183’196’206’215’225’235’246’254’26