NEET UG · Biology · Class 12
Biotechnology: Principles and Processes: NEET previous year questions
46 questions in ten years of NEET, asked in 10 of 10 years. Here is what this chapter tests and how.
- Asked in 10 years
- 46
- 10 of 10 papers
- 10-year average
- 4.4
- a paper · 4.8 since 2024 +0.6
- Typical range
- 3–6
- questions a paper; fewest 1, most 6
- Weighted estimate
- 4.9
- model, recent papers count more · about 19 marks
- Typical range (fact)
- 10-year average (fact)
- Weighted estimate (model)
Typical range: questions per paper in the middle eight of the ten papers, leaving out the single highest and lowest year (about the 10th to 90th percentile), each paper scaled to 180 questions. The ten-year average and the range are historical fact. The weighted estimate is a model: each paper's share, with recent papers counting more (half-life three years), and dropped syllabus removed. It is a guide to effort, not a forecast.
What to do
How to spend time on Biotechnology: Principles and Processes
Priority
Among the chapters that held the first half of all NEET questions in ten years. How bands are set
- Past questions
- 46in 10 of 10 papers
- Study plan gives it
- 27 hover 12 months; 20 h over 6
Ideas asked most often
- Origin of Replication (ori)4×
- Bioreactors3×
- DNA Precipitation with Chilled Ethanol3×
- UV Visualization of DNA3×
- Palindromic Sequences3×
190 of the 239 ideas in Lumi’s map of this chapter have not been asked or touched in ten years. New questions often come from ideas like these, so learn the whole chapter, not just the list above.
Questions per year
Kind of question
SHARE OF 10 YEARS- Recall52%
- Concept17%
- Two statements9%
- Figure-based7%
- Application4%
- Match the lists4%
- Assertion–reason4%
- Calculation2%
Most common asks: definition recall (6); identify recognition sequence of enzyme (3); order of steps in a process (3).
Profile
This chapter in numbers
Where it ranks, how steady it is, and what it costs to study.
- Rank, all NEET chapters
- #2
- of 80 by weighted estimate
- Rank in Biology
- #2
- of 32 chapters
- Share of the paper
- 2.5%
- ten-year average
- Busiest year
- 2020
- 6 questions
- Year to year
- Some swing
- varies by about 1.4 questions
- Long-run direction
- +0.17
- questions per paper, per year
- Easy questions
- 70%
- 18% of ideas rated hard
- With a figure
- 7%
- graph, diagram or structure
- Botany / Zoology
- 13 / 33
- questions by section
- Study time
- 27 h
- 20 h on a 6-month plan
- Marks per study hour
- 0.71
- #6 of 80 chapters
- Foundation for others
- 57%
- of its ideas are used in other chapters
Difficulty, year by year
Each question rated easy, medium or hard against NCERT.
- 20175
- 20181
- 20193
- 20206
- 20216
- 20225
- 20235
- 20245
- 20256
- 20264
- Easy
- Medium
- Hard
What it asks you to do
SHARE OF QUESTIONS- Recall61%
- Concept26%
- Application7%
- Figure reading4%
- Multi-step2%
Coverage
How much of the chapter NEET has used
We split the chapter into 239 ideas. In ten years NEET built a question around 28 of them. Most of the chapter has never been asked, which is why learning it whole beats memorising past questions.
- Asked as the main idea
- 28
- Touched on in a question
- 21
- Never asked
- 190
Topics
What repeats, topic by topic
Questions per topic in each year's paper. Topics that come every year are worth learning first.
- Restriction Enzymes · Every year22324333121
- Cloning Vectors · Every year18212112432
- Polymerase Chain Reaction · Every year612111
| Topic | 2017 | ’18 | 2019 | ’20 | 2021 | ’22 | 2023 | ’24 | 2025 | ’26 | Total |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Restriction EnzymesEvery year | 3 | · | 2 | 4 | 3 | 3 | 3 | 1 | 2 | 1 | 22 |
| Cloning VectorsEvery year | 2 | · | 1 | 2 | 1 | 1 | 2 | 4 | 3 | 2 | 18 |
| Polymerase Chain ReactionEvery year | · | 1 | · | · | 2 | 1 | · | · | 1 | 1 | 6 |
Restriction Enzymes
Expect a one-line factual recall item about Restriction Enzymes; a conceptual single-answer item (reasoning rather than recall) is the next most likely shape. Past framings centre on: reagent identification; identify recognition sequence of enzyme.
Cloning Vectors
Expect a one-line factual recall item about Cloning Vectors; a conceptual single-answer item (reasoning rather than recall) is the next most likely shape. Past framings centre on: definition recall; identify labelled part in diagram.
Drill down
Topic, idea, question
Open a topic to see every idea NEET tested inside it, then open an idea to read the questions. Each row shows its ten papers.
16 ideas inside
- Repeated3 · 14%
- Repeated3 · 14%2
- Repeated3 · 14%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
- 1 · 5%
Squares are the ten papers, 2017 to 2026; darker means more questions. Share is of the level above.
Ideas
The exact ideas NEET has asked
Inside the topics, 32 different ideas were tested. These came more than once, or are new since 2024.
- Origin of Replication (ori)4×
identify vector feature by function
- Bioreactors3×
identify equipment for industrial process
- DNA Precipitation with Chilled Ethanol3×
identify what a reagent precipitates
- UV Visualization of DNA3×
reagent identification
- Palindromic Sequences3×
identify palindromic recognition sequence
- Exponential DNA Amplification2×
assertion-reason on biotechnology technique
- Ti Plasmid and T-DNA2×
match organism with biotech use
- DNA Denaturation Step2×
order of steps in a process
- Primer Annealing StepNew1×
identify pcr step by event
- Enzymatic Cell Lysis for DNA IsolationNew1×
assertion-reason on rdna technology
- Definition of a PlasmidNew1×
statement i/ii on plasmids
- Anode Migration in ElectrophoresisNew1×
statement i/ii on gel electrophoresis
- Role of DNA LigaseNew1×
count correct statements on cloning enzymes
- Blue-White Screening OutcomesNew1×
statement i/ii on recombinant screening
- Recombinants as White ColoniesNew1×
identify recombinant selection strategy from plasmid map
- Hind II Cleavage PatternNew1×
identify recognition sequence of enzyme
Every question
Biotechnology: Principles and Processes questions, year by year
The opening words of each question as it appeared in the official paper.
NEET 20264 questions
- Q162During PCR, primers bind to the DNA strands in the ___ step.One-line MCQ
- Q168Given below are two statements: Statement I: Plasmids are autonomously replicating DNA. Statement II: Plasmids are extrachromosomal DNA.…Two statements
- Q176Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R. Assertion A: In recombinant DNA…Assertion–reason
- Q180Match List-I with List-II. List-I | List-II A. Transformation | I. Restriction enzyme B. Cloning site | II. Transfer DNA to host bacteria…Match the lists
NEET 20256 questions
- Q92Polymerase chain reaction (PCR) amplifies DNA following the equation.One-line MCQ
- Q115In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to…Figure-based · figure
- Q126Identify the part of a bio-reactor which is used as a foam braker from the given figure.Figure-based · figure
- Q136Given below are two statements : Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of…Two statements
- Q172Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase…Other
- Q179The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the…Two statements
NEET 20245 questions
- Q106Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:One-line MCQ
- Q133What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would…Other
- Q165Which of the following statements is incorrect?One-line MCQ
- Q169The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:Figure-based · figure
- Q180The 'Ti plasmid' of Agrobacterium tumefaciens stands for:One-line MCQ
NEET 20235 questions
- Q107Upon exposure to UV radiation, DNA stained with ethidium bromide will showOne-line MCQ
- Q121During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates outOne-line MCQ
- Q127In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.One-line MCQ
- Q141Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA…Other
- Q158Which of the following is not a cloning vector?One-line MCQ
NEET 20225 questions
- Q102Which one of the following statement is not true regarding gel electrophoresis technique ?One-line MCQ
- Q111Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : Polymerase…Assertion–reason
- Q141In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?One-line MCQ
- Q169Given below are two statements : Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic…Two statements
- Q199Which of the following is not a desirable feature of a cloning vector ?One-line MCQ
NEET 20216 questions
- Q101During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :One-line MCQ
- Q107Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?One-line MCQ
- Q123DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :One-line MCQ
- Q147Plasmid pBR322 has PstI restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for…One-line MCQ
- Q159A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :One-line MCQ
- Q164During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the…One-line MCQ
NEET 20206 questions
- Q7Choose the correct pair from the following :One-line MCQ
- Q18In gel electrophoresis, separated DNA fragments can be visualized with the help of :One-line MCQ
- Q21The specific palindromic sequence which is recognized by EcoRI is :One-line MCQ
- Q22Identify the wrong statement with regard to Restriction Enzymes.One-line MCQ
- Q27Match the organism with its use in biotechnology. (a) Bacillus thuringiensis | (i) Cloning vector (b) Thermus aquaticus | (ii)…Match the lists
- Q78The sequence that controls the copy number of the linked DNA in the vector, is termed :One-line MCQ
NEET 20193 questions
- Q113Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes ?One-line MCQ
- Q122DNA precipitation out of a mixture of biomolecules can be achieved by treatment with :One-line MCQ
- Q145Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.One-line MCQ
NEET 20181 question
- Q101The correct order of steps in Polymerase Chain Reaction (PCR) isOne-line MCQ
NEET 20175 questions
- Q94What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?One-line MCQ
- Q115The process of separation and purification of expressed protein before marketing is called :One-line MCQ
- Q125DNA fragments are :One-line MCQ
- Q132A gene whose expression helps to identify transformed cell is known as :One-line MCQ
- Q145The DNA fragments separated on an agarose gel can be visualised after staining with :One-line MCQ
Questions from the official NEET UG papers published by NTA (CBSE before 2019). Topic and idea tags, counts and notes are Lumi’s.