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Friday, 9 October

NEET UG 2021 · Zoology · Question 164

NEET 2021 Zoology, question 164

Question 164 of the NEET 2021 paper (code O1) is a Zoology question from Biotechnology: Principles and Processes. Its idea, DNA Denaturation Step, was asked twice, both in 2021, across NEET 2017–2026. The NTA final answer key gives option 1.

Updated 2026-10-08

Q164 · Zoology

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?

  1. 1DenaturationOfficial answer
  2. 2Ligation
  3. 3Annealing
  4. 4Extension

Official answer: option 1 · NTA final answer key

Same idea

DNA Denaturation Step, in other papers

NEET asked this idea twice, both in 2021. The other question below.

  • 2021 Q107Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?One-line MCQ · Easy

DNA Denaturation Step: every question and how it was framed

The chapter

Biotechnology: Principles and Processes in ten years of NEET

46 questions from 2017–2026, asked in 10 of 10 papers, about 4.4 a paper. Polymerase Chain Reaction alone had 6.

Biotechnology: Principles and Processes: questions per paper

0365’171’183’196’206’215’225’235’246’254’26