NEET UG 2021 · Botany · Question 147
NEET 2021 Botany, question 147
Question 147 of the NEET 2021 paper (code O1) is a Botany question from Biotechnology: Principles and Processes. Its idea, PvuI/PstI Site Insertional Inactivation, was asked only this once in NEET 2017–2026. The NTA final answer key gives option 3.
Updated 2026-10-08
Q147 · Botany
Plasmid pBR322 has PstI restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain
- 1it will lead to lysis of host cell.
- 2it will be able to produce a novel protein with dual ability.
- 3it will not be able to confer ampicillin resistance to the host cell.Official answer
- 4the transformed cells will have the ability to resist ampicillin as well as produce -galactoside.
Official answer: option 3 · NTA final answer key
Same idea
The only question on PvuI/PstI Site Insertional Inactivation
No other NEET paper from 2017–2026 tested this exact idea.
PvuI/PstI Site Insertional Inactivation: every question and how it was framed
The chapter
Biotechnology: Principles and Processes in ten years of NEET
46 questions from 2017–2026, asked in 10 of 10 papers, about 4.4 a paper. Cloning Vectors alone had 18.