Biology · Class 12 · Chapter 10
Biotechnology and its Applications
12 lessons 89 min 2 simulations
This chapter takes the tools of recombinant DNA technology out of the lab and into farms, hospitals and law courts. It covers tissue culture and somatic hybrids, GM crops such as Bt cotton and RNAi-protected tobacco, recombinant insulin, gene therapy for ADA deficiency, molecular diagnosis, transgenic animals, and the ethics of GM organisms and biopiracy.
What the exam asks
NEET asks why Bt toxin spares the bacterium (inactive protoxin, activated by alkaline gut pH), which cry gene controls which pest, how RNAi silences nematode mRNA, how Eli Lilly made insulin (A and B chains separately in E. coli, C peptide absent), the ADA gene-therapy steps, PCR, probes and ELISA in diagnosis, the uses of transgenic animals with Rosie's 2.4 grams per litre, and GEAC, biopiracy and the Basmati patent. Marks are lost by saying the toxin is activated by acid, by mixing up cryIAb and cryIAc, and by forgetting that ADA gene therapy with lymphocytes is not permanent.
Lessons
12 lessons · 89 min
1Three research areasNCERT §10 (chapter introduction)6 min2Food production and tissue cultureNCERT §10.16 min3Meristem culture and somatic hybridsNCERT §10.17 min4Genetically modified cropsNCERT §10.16 min5Bt cottonNCERT §10.19 min 1 sim6RNA interferenceNCERT §10.110 min 1 sim7Genetically engineered insulinNCERT §10.2, §10.2.17 min8Gene therapyNCERT §10.2.26 min9Molecular diagnosisNCERT §10.2.36 min10Transgenic animalsNCERT §10.36 min11Ethical issues and biopiracyNCERT §10.47 min12Chapter reviewMust-know facts, traps and formulas13 min